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MS培養基

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names：
MS Medium
CAS號：

MDL Number： NA
MF(分子式)： NA MW(分子量)： NA
EINECS: Reaxys Number:
Pubchem ID: Brand:BIOFOUNT

MS培養基，全稱Murashige and Skoog培養基是一種廣泛使用的植物組織培養生長培養基。MS培養基含有大量營養素，其中包括高水平的硝酸鹽和有機添加劑，例如瓊脂，糖，維生素和生長調節劑。經常添加到MS培養基的重要生長調節劑包括IAA（生長素/形態發生素）和Kinetin（細胞分裂素/細胞分裂啟動子）。

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中文別名	MS培養基;MS 培養基;MS-培養基
英文別名	MS Medium,MS-Medium,
CAS號
Inchi	Not available
InchiKey	Not available
分子式 Formula	NA
分子量 Molecular Weight	NA
溶解度Solubility
性狀	Solid,固體粉末
儲藏條件 Storage conditions	Store at room temperature；常溫陰涼處存儲

MS培養基詳細介紹：
MS培養基全稱為Murashige和Skoog培養基（MS）最初由Murashige和Skoog于1962年制作成功，旨在優化煙草愈傷組織生物測定系統，以促進細胞分裂素的研究。從那時起，MS培養基被廣泛用于微繁殖，器官培養，愈傷組織培養和懸浮培養。該配方是無機的營養混合物鹽，維生素和氨基酸。Murashige和Skoog培養基（MS培養基）提供了所有必需的宏觀元素和微量元素。磷酸二氫鉀是磷酸鹽的來源。微量元素如硼，錳，鉬，銅和鋅在植物代謝中起著至關重要的作用。硼在碳水化合物代謝中起關鍵作用。硫胺素，煙酸，吡ox醇，肌醇是植物糖酵解和三環素循環以及植物的初次和二次代謝等普遍途徑中的酶輔因子。甘氨酸是氨基酸的來源。
該產品經過植物組織培養測試，但用戶應獨自負責確保培養基對單個物種的適用性。

MS培養基植物組織培養試驗
The growth promoting properties of medium is assessed by providing plant cultures with relative humidity of about 60%±2%,temperature 22ºC±2ºC and photoperiod of about 16:8.The plant species showed actively growing callus and shoots with no structural, necrotic and toxic deformity.

MS培養基實驗注意事項：
1.實驗前需戴好防護眼鏡，穿戴防護服和口罩，佩戴手套，避免與皮膚接觸。
2.實驗過程中如遇到有毒或者刺激性物質及有害物質產生，必要時實驗操作需要手套箱內完成以免對實驗人員造成傷害。
3.取樣品的移液槍頭需及時更換，必要時為避免交叉污染盡可能選擇濾芯吸頭。
4.稱量藥品時選用稱量紙，并無風處取藥和稱量以免揚撒，試劑的容器使用前務必確保干凈，并消毒。
5.取藥品時盡量采用多個藥勺分別使用，使用后清洗干凈。
6.實驗后產生的廢棄物需分類存儲，并交于專業生物廢氣物處理公司處理，以免造成環境污染。
大規格定制：定制產品請將信息發送至sales@bio-fount.com。
Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.

產品說明	MS培養基（MS medium）用于植物組織培養，MS培養基成分,MS培養基使用注意事項,MS培養基實驗等見說明
Introduction	MS medium（MS培養基）Murashige and Skoog Medium (MS) provides all the essential macroelements and microelements. Potassium dihydrogen phosphate serves as a source of phosphate.
Application1	MS培養基的用途:用于植物組織培養的基礎培養基
Application2
Application3

MS培養基成分mg / L：
MS培養基中的大量元素
硝酸銨1650.000
氯化鈣332.200
硫酸鎂180.690
硝酸鉀1900.000
一元磷酸鉀170.000
MS培養基中的微量元素：
硼酸 6.200
六水合氯化鈷 0.025
五水合硫酸銅 0.025
EDTA二鈉二水合物鹽37.300
七水硫酸亞鐵 27.800
一水硫酸錳 16.900
鉬酸（鈉鹽） 0.213
碘化鉀 0.830
七水合硫酸鋅 8.600
MS培養基中的維生素：
肌醇 100.000
煙酸（游離酸） 0.500
鹽酸吡哆醇 0.500
鹽酸硫胺素 0.100
MS培養基中的氨基酸
甘氨酸 2.000
總計（克/升） 4.4

使用MS培養基注意事項：
•添加之前，確保培養基的pH合適膠凝劑，因為酸性pH值會導致膠凝減少產生半固體流動的凝膠，而堿性pH值會導致形成硬化的凝膠。
•使用蒸餾水/組織培養級水是建議用于自來水或更低的介質制備等級的水可能導致鹽分沉淀和不合適凝膠化。
•避免制備濃縮液，因為這會導致沉淀鹽。

MS培養基使用指導：
•通過添加所需量的粉末占總體積的三分之二，保持恒定，溫和攪拌直至培養基完全溶解。
•高壓滅菌前添加熱穩定劑
•用蒸餾水補足最終培養基所需的體積。
•使用1M NaOH溶液 /將培養基的pH調節至5.75±0.5鹽酸。
•加入膠凝劑并將介質加熱至沸騰膠凝劑完全溶解。
•通過在15 lbs和121°C下高壓滅菌來滅菌培養基15分鐘。
•添加熱不穩定產品前，需將高壓滅菌的介質冷卻至約45°C熱。
•在無菌條件下分配所需量的培養基無菌培養皿中的層流氣流單元。

MS Medium Description : Murashige and Skoog Medium (MS) was originally formulated by Murashige and Skoog in 1962 to optimize tobacco callus bioassay system for facilitating the study of cytokinins. Since then, it is widely used for micro propagation, organ culture, callus culture and suspension culture. The formulation is a nutrient blend of inorganic salts, vitamins and amino acid. Murashige and Skoog Medium (MS) provides all the essential macroelements and microelements. Potassium dihydrogen phosphate serves as a source of phosphate. Microelements like Boron, Manganese, Molybdenum, Copper and Zinc play vital role in plant metabolism. Boron plays a key role in carbohydrate metabolism. Thiamine, nicotinic acid, pyridoxine, inositol act as enzymatic cofactors in universal pathways including glycolysis and TCA cycle along with primary and secondary metabolism in the plants. Glycine serves as a source of amino acid.

警示圖
危險性	Warning
危險性警示	Not available
安全聲明	H303吞入可能有害+H313皮膚接觸可能有害+H333吸入可能對身體有害
安全防護	P264處理后徹底清洗+P280戴防護手套/穿防護服/戴防護眼罩/戴防護面具+P305如果進入眼睛+P351用水小心沖洗幾分鐘+P338取出隱形眼鏡（如果有）并且易于操作,繼續沖洗+P337如果眼睛刺激持續+P313獲得醫療建議/護理
備注	實驗過程中防止吸入、食入、穿戴好個人防護用品

Plant tissue culture for biotechnology Prakash P. Kumar, Chiang Shiong Loh, in Plant Biotechnology and Agriculture, 2012

Somatic (asexual) procedures (haploids, protoplasts, cell selection) and their applications Tanya Tapingkae, ... Acram Taji, in Plant Biotechnology and Agriculture, 2012

Plant Genetic Engineering Towards the Third Millennium R. García, ... M. García, in Developments in Plant Genetics and Breeding, 2000

Immobilized Cells J.-N. Barbotin, ... D. Thomas, in Progress in Biotechnology, 1996

Spinach S.C. PANDEY, G. KALLOO, in Genetic Improvement of Vegetable Crops, 1993

Plant tissue culture for biotechnologyPrakash P. Kumar, Chiang Shiong Loh, in Plant Biotechnology and Agriculture, 2012.

Summary:Plant tissue culture involves excising plant tissues and growing them on nutrient media. It is used rather broadly to include several variations, such as meristem culture for propagation of virus-free plants, protoplast culture, cell suspension culture, tissue and organ culture, and anther or pollen culture for producing haploid plants. This chapter focuses on various technical aspects of plant tissue culture. A suitable explant is selected and prepared for culture, and later incubated on an appropriate nutrient medium for growth and differentiation. The basic laboratory setup, handling of explant tissue, nutrient medium and establishing the culture, and incubation of cultures are also discussed in this study. A laboratory that can handle plant biochemistry or physiology-type experiments meets most of the general requirements of plant tissue culture. It is a valuable tool for research on morphogenesis, cell signaling, physiology, and molecular biology, as well as crop improvement by biotechnology. The implications of plant tissue culture technology for agricultural biotechnology is immense.

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